Collectively, the data suggest that CCRK positively regulates the kinetics by which ciliary proteins such as Smoothened and Gli2 are imported into the cilium, and that the efficiency of ciliary recruitment allows for potent responses to Hedgehog signaling over long time periods. Ccrk mutant cells exhibit defects in intraflagellar transport (the transport mechanism used to assemble cilia), as well as slowed kinetics of ciliary enrichment of key Hh pathway regulators.
Similar to Chlamydomonas mutants lacking the CCRK homolog, LF2, mouse Ccrk mutant cells show defective regulation of ciliary length and morphology. In vitro experiments indicate that Ccrk mutant cells show a greater deficit in response to signaling over long time periods than over short ones. Cell biological, biochemical and genetic analyses indicate that CCRK is required to control the Hedgehog pathway at the level or downstream of Smoothened and upstream of the Gli transcription factors, Gli2 and Gli3. Mice mutant for Ccrk exhibit a variety of developmental defects indicative of inappropriate regulation of this pathway. Here, we investigate the role of Cell Cycle Related kinase (CCRK) in regulation of cilium-dependent Hh signaling in the mouse. Cells require a small organelle, the primary cilium, to respond properly to Hh signals and the key regulators of Hh signal transduction exhibit dynamic localization to this organelle when the pathway is activated. Control charts - X-bar, R-chart, S-chart, IMR-chart, P-chart, C-chart, U-chart, CUSUM-chart.The Hedgehog (Hh) signaling pathway plays a key role in cell fate specification, proliferation, and survival during mammalian development.Quantile-quantile Q-Q plots for different distributions.Bland-Altman plot with multiple measurements per subject.Sampling (random, periodic, conditional).Report includes: AUC (with confidence intervals), curve coordinates, performance indicators - sensitivity and specificity (with confidence intervals), accuracy, positive and negative predictive values, Youden's J (Youden's index), Precision-Recall plot. Receiver operating characteristic curves analysis (ROC analysis).ĪUC methods - DeLong's, Hanley and McNeil's.LD values (LD50/ED50 and others), cumulative coefficient calculation. Kaplan-Meier (log rank test, hazard ratios).Unit root tests - Dickey–Fuller, Augmented Dickey–Fuller (ADF test), Phillips–Perron (PP test), Kwiatkowski–Phillips–Schmidt–Shin (KPSS test).Tests for heteroscedasticity: Breusch–Pagan test (BPG), Harvey test, Glejser test, Engle's ARCH test (Lagrange multiplier) and White test.Stepwise (forward and backward) regression.
Weighted least squares (WLS) regression.Multivariate linear regression (residuals analysis, collinearity diagnostics, confidence and prediction bands).Wilcoxon Matched Pairs Test, Sign Test, Friedman ANOVA, Kendall's W (coefficient of concordance). Mann-Whitney U Test, Kolmogorov-Smirnov test, Wald-Wolfowitz Runs Test, Rosenbaum Criterion. Rank correlations (Kendall Tau, Spearman R, Gamma, Fechner).2x2 tables analysis (Chi-square, Yates Chi-square, Exact Fisher Test, etc.).Within subjects ANOVA and mixed models.Post-hoc comparisons - Bonferroni, Tukey-Kramer, Tukey B, Tukey HSD, Neuman-Keuls, Dunnett.One-way and two-way ANOVA (with and without replications).Multiple definitions for computing quantile statistics.Frequency tables analysis (for discrete and continuous variables).Normality tests (Jarque-Bera, Shapiro-Wilk, Shapiro-Francia, Cramer-von Mises, Anderson-Darling, Kolmogorov-Smirnov, D'Agostino's tests).Migration guide for users switching from Analysis ToolPak. Options to emulate Excel Analysis ToolPak results and.
Measure frequency of values in statplus license#
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